DEFINING SUBSTRATE SPECIFICITY IN TRYPTOPHAN SYNTHASE BETA‐SUBUNIT HOMOLOGS
نویسندگان
چکیده
منابع مشابه
Tryptophan Synthase Uses an Atypical Mechanism To Achieve Substrate Specificity.
Tryptophan synthase (TrpS) catalyzes the final steps in the biosynthesis of l-tryptophan from l-serine (Ser) and indole-3-glycerol phosphate (IGP). We report that native TrpS can also catalyze a productive reaction with l-threonine (Thr), leading to (2S,3S)-β-methyltryptophan. Surprisingly, β-substitution occurs in vitro with a 3.4-fold higher catalytic efficiency for Ser over Thr using saturat...
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Early in this study, an α-Amylase from Bacillus megaterium WHO (BMW) was isolated from hot springs of Ramsar (North of Iran), and its gene was cloned in E.coli. Based on its conserved sequence regions and substrate specificity, it was classified as intermediary group enzymes with the specificity of oligo-1,6-glucosidase and neopullulanase subfamilies. In the sixth conserved re...
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early in this study, an α-amylase from bacillus megaterium who (bmw) was isolated from hot springs of ramsar (north of iran), and its gene was cloned in e.coli. based on its conserved sequence regions and substrate specificity, it was classified as intermediary group enzymes with the specificity of oligo-1,6-glucosidase and neopullulanase subfamilies. in the sixth conserved region (83-qvngiwmmp...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2007
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.21.6.a1018-c